Transformation Protocol (C2528)

由 A Froger 著作 · 2007 · 被引用 594 次 — coli using the heat shock method is a basic technique of molecular biology. It consists of inserting a foreign plasmid or ligation product into bacteria. This ...

由 AY Chang 著作 · 被引用 177 次 — Calcium chloride heat-shock transformation is a powerful molecular biology technique used to introduce foreign DNA into a host cell.

Place transformation tube into a 42°C water bath for 42 seconds (30-60 sec).

The protocols for preparing competent cells vary by choice of transformation method—heat shock or electroporation. Heat shock transformation is also known as ...

Heat Shock: Both the temperature and the timing of the heat shock step are important and specific to the transformation volume and vessel. Using the ...

2017年11月13日 — Procedure · Take competent cells out of -80°C and thaw on ice (approximately 20-30 mins). · Remove agar plates (containing the appropriate ...

Heat-shock the cells for 45 seconds at 42°C without shaking. ... Each lot of Stbl3™ competent cells is tested for transformation efficiency using the control ...

2012年5月21日 — Heat shock at 42°C for 30 seconds*. Do not mix. Add 950 µl of room temperature media* to the tube. Place tube at 37°C for 60 minutes. Shake ...